DOI: 10.36347/sajp.2022.v11i10.002

Pages: 182-186

Purpose: To study the effect of isoquercitrin on cell apoptosis and cycle of HepG2 cells. Materials and Method: Different concentrations of isoquercitrin were used to act on HepG2 cells, and the effect of isoquercitrin on the proliferation of HepG2 cells was detected by CCK8. Cell morphology and growth were observed under inverted microscope. Flow cytometry was used to detect the apoptosis and cycle changes of HepG2 cells. Results: CCK8 found that isoquercitrin inhibited the growth of HepG2 cells, and it was correlated with the concentration and action time of isoquercitrin. Under the inverted microscope, it was observed that the number of cell survival gradually decreased with the increase of concentration or time of isoquercitrin acted on HepG2 cells. Flow cytometry showed that with the increase of isoquercitrin concentration, the number of cells blocked in S phase gradually decreased and the number of cells blocked in G2/M phase gradually increased. Conclusion: Isoquercitrin can induce apoptosis of HepG2 cells and interfere with S phase and G2/M phase in cell cycle.