DOI: 10.36347/sajp

Pages: 1-4

A simple, rapid, precise and accurate UV Spectrophotometric method was developed and validated for the estimation of Lamivudine in commercial tablets using two different solvent blends viz. Methanol: Water: 0.1N HCl (3:1:1) and Methanol: Water: 0.1N NaOH (3:1:1) . Absorption maxima were determined and found 282 nm and 272 nm in each respective solvent blends followed by linearity range determination which was found within the range of 1-22 µg/ml. From the linearity range, calibration curve is prepared in the range of 2-10 µg/ml and validated the various parameters in bulk and formulations. All the experiments were carried out in triplicate, for each parameter relative standard deviation and % recoveries was calculated and the data were statistically interpreted and were found to be significant and within the limit. The proposed methods are further evaluated for precision, robustness studies. The results indicate the methods were precise and robust.