DOI: 10.36347/sjams.2019.v07i05.058

Pages: 1977-1984

In search of possible, safe and toxic anticancer drug for the treatment of breast cancer, molecular pathological study of the activities of Citrus limon juice on MCF-7 cell line induced breast cancer in Sprague Dawley rats compared to Tamoxifen was carried, over one hundred and twenty Sprague dawley rats of 40 days old, average body weight 180-220g were divided into ten (10) containing of 12 animals per group, group 1 was control, fed only with rat chow and water, group 2 was MCF-7 cell line induced rat alone (BCIR only), group 3 Citrus limonj uice (CLJ) at 8.88%, Group 4 Citrus limon juice(CLJ) at 17.32%, group 5 Citrus limon juice(CLJ) at 25.98%, group 6 was given 0.2mg/kg of Tamoxifen alone, group 7 (BCIR+CLJ at 8.88%), group 8 (BCIR+CLJ at 17.32%), group 9 (BCIR+CLJ at 25.98%) and group (BCIR+ 0.2mg/kg of Tamoxifen). Af the end of the administration, animals were sacrificed, fresh blood were taken into the hepharinizsed container for heamatological analysis such as RBC, MCV, MCHC, PCV, WBC, Hb, Eosinophil, Basophil, Lymphocyte, Monocyte and Platelet counts, while the other part of the blood were centrifuged at 3000 rpm, serum collected and stored appropriately for Biochemical studies, Heamatological profile revealed significant reduction in the red cell indices, Breast cancer induced rat group at p<0.001 compared to the Breast cancer induced rat treated with Citrus limon and Breast cancer induced rats group treated with Tamoxifen while other non- Breast cancer induced group were not significant. There was significant increase in WBC cells and differential respectively in the Breast cancer induced group at p>0.001 when compared to all the Breast cancer induced post treated with Citrus limon and Breast cancer induced group pos-treated with Tamoxifen simultaneously. In conclusion, this study showed that Citrus limon juice exhibit anticancer activities reverting the abnormal blood indices thereby preventing possible pathological conditions on the body du