DOI: 10.36347/sajp

Pages: 41-46

A simple, and sensitive validated GCMS-EI analytical method was developed for the determination of dithiocarbamates (Mancozeb and Metiram) residues in different aquatic tox mediums. The tox mediums were those which provide nutrients and help the growth of different aquatic organisms for their survival and multiplication. The constituent of different mediums includes blended water for fish, M4 Medium for Daphnia magna, OECD TG 201 medium for Alga and 20XAAP Medium for lemna. The dithiocarbamates residue involves the reduction of ditioncarbonate moiety under strong acidic conditions in presence of stannous chloride (SnCl2) as reducing agent, evolution of carbon disulfide (CS2) insitu extraction of the CS2 into a layer of iso-octane subsequently analysis of CS2 content by GC–MS in SIM mode. The established optimum conditions provided 5.0 min retention for CS2 and the total time of chromatographic analysis was 7.0 min. The linear graph has the lowest detection 0.01 mg/L. The lowest limit of quantification was 0.03 mg/L. The method was validated at levels of 0.03 and 0.3 mg/L. Recovery spiked samples of mancozeb and Metiram in different mediums were in the range 91-98%, with relative standard deviations 0.78 to 2.2 % (n=5). The proposed method can be applied successfully for the determination of dithiocarbamate residues in different aquatic solutions