DOI: 10.36347/sjams.2019.v07i06.029

Pages: 2177-2180

Purpose –Surgical site infection is one of the common post-operative complications. Microorganisms with emerging drug resistance being one of the major contributing factors, extended spectrum beta lactamase (ESBL) producing Gram Negative Bacilli pose a challenge in the treatment of surgical site infections. The present study is undertaken to study the prevalence of the ESBL production among various Gram Negative Bacilli isolated from surgical site infections at a tertiary care hospital. Material & Methods: A cross-sectional study during May 2018 to December 2018 including 112 samples from infected surgical wounds. A total of 73 bacterial isolates were identified by standard microbiological identification tests and Antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method following CLSI guidelines 2018. Gram negative isolates which were resistant to third generation cephalosporins were screened for ESBL production by double disc diffusion method and confirmation was done by Combination disc method. Results: Among 112 samples from infected surgical wounds, 73 (65.17%) were culture positive with 42 (57.53%) Gram positive isolates and 31(42.46%) Gram negative isolates. Gram positive isolates included 32 Staphylococcus aureus and 10 Coagulase negative staphylococci (CoNS). Gram negative bacilli included 14 Klebsiella species, 10 Escherichia coli, 4 Pseudomonas aeruginosa, 2 Proteus species and 1 Citrobacter freundii. ESBL production was detected in 18 (58.06%) Gram negative bacilli by Double disc diffusion method and confirmed by Combination disc method in 12 (38.7%) Gram negative bacterial isolates. Conclusion: A significant number of ESBL producing Gram negative bacteria were detected from surgical site infections which indicate the necessity of developing and implementing antibiotic policy.