DOI: 10.36347/sjams.2019.v07i07.006

Pages: 2284-2288

Background: Human Papilloma Virus is one of the most common sexually transmitted infections. HPV is usually harmless and goes away by itself, but some types can lead to cancer or genital warts. All womens are at risk for cervical cancer it occurs most often in women over age 30. This study aimed to investigate HPV infection in women with cytological and/or colposcopic abnormalities in Uttarakhand population and also verify the performance of MY09/MY11 primers for HPV detection. Materials and Methods: For this proposed study we collected the clinical specimens, and the study was conducted from the samples collected from Shri Mahant Indiresh hospital, Dehradun which includes OPD and IPD of gynaecology and obstetrics. Results: The average age of the studied population was 36 years (range: 18-58years). Cytological results, 03 (3.12%) cases ASCUS, 45 (46.87%), NILM, 28 (29.16%) cases inflammatory smear, 04 (4.16%), Bacterial vaginosis, 02 (2.08%), Atrophic vaginitis, 03 (3.12%), Unsatisfactory smear, 01 (1.04%) cases Post-menopausal smear & 06(6.25%) cases HSIL for squamous cell carcinoma showed positive colposcopy. Only 06 (6.5%) patient had HSIL and a positive sample to HPV, which demonstrated the effectiveness of PCR as standard molecular method. Discussions and Conclusion: This study suggests that more than one type of oligonucleotide primer should be used in clinical samples to increase the sensitivity in HPV detection. For the proper management and follow up protocols for the cases with positive HPV infections, such studies are clinically, relevant.