DOI: 10.36347/sjams.2019.v07i07.052

Pages: 2537-2539

Background: D antigen is highly immunogenic even weak D is also potent enough to cause hemolytic transfusion reaction so it is necessary to detect the weak D, and should consider as Du positive as donor and Du negative as recipient for safe clinical transfusion practice. Method: Rh- typing has done by antisera IgM anti-D monoclonal antisera D1, blend of IgM and IgG monoclonal antisera D2…. (Tulip diagnostic) by slide and immediate spin tube method and Gel card with AHG antisera. Result: total 17717 donor blood sample were tested and it is observed that Prevalence of Rh-negative donor is 1091(6.15%) and prevalence of weak Du positive among total donor is 0.01% while it is 0.18% among Rh negative donor. Conclusion: The prevalence of weak D u is very low and comparison with other author is difficult due lack of standard set of technique and reagent. Even though red cell with weak Du are less immunogenic than Rh negative all donor must tested for Du testing should implement in routine blood bank practice for safe transfusion practice.