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Scholars Journal of Applied Medical Sciences | Volume-7 | Issue 07
Pretransfusion Compatibility Testing by Conventional Tube and Gel Method A Comparative Study at Tertiary Care Center
Gautam Chauhan, Bharti Parghi
Published: July 30, 2019 | 45 82
DOI: 10.36347/sjams.2019.v07i07.067
Pages: 2604-2606
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Abstract
Introduction: pre-transfusion compatibility testing is critical in transfusion medicine so as to prevent immune reaction which might be fatal sometime. The traditional tube method is gold standard however newly introduce matrix gel card has improve the quality of testing and give end point stable result. Aim: This study done to compare newly introduce matrix gel card method in our blood bank to conventional tube method. Material and method: Total 1200 random sample taken for compatibility testing by commonly use test tube method and then matrix gel card method based on indirect Coomb’s test. Result: Our study show 100% compatibility by saline based CTT, while 05 (0.4%) samples were incompatible by CTT with anti-human globulin (AHG) and matrix gel card. So 05 sample show false positive and 05 sample show false negative of previous 100% compatible by CTT without AHG. Sensitivity and specificity is 100% of matrix gel card and indirect Coomb’s tube test using AHG, whereas saline tube test specificity is 99.5%. Conclusion: Matrix gel card method is simple, easy to performer and gives more stable end point result that can be recorded and photocopied. It is more sensitive and specific than Conventional saline method though CTT with AHG is sensitive and specific as Gel card but result cannot record and require more time than saline and gel card method.