DOI: 10.36347/sjams.2019.v07i12.062

Pages: 4140-4146

Background: HPV infection is more frequent and more persistent in HIV+ women; this increases the risk of invasive cervical cancer that would progress about 10 years earlier in HIV+ women than in HIV-women. This data argues for accurate molecular epidemiology of genotypes and early detection of high-risk HPV infections for effective cervical cancer prevention. Objective: To genotype HPV with molecular biology technique and to evaluate the socio-demographic and clinical factors favoring HPV infection among women living with HIV (WLHIV). Materials and Methods: The molecular identification of 28 HPV genotypes (19 HPV-HR and 9 HPV-LR) was performed on endocervical samples using the Seegene AnyplexTM II HPV 28 Detection using the Bio CFX96 of Biorad. Data analysis was performed by the R studio software (version 3.6.0). Proportion comparisons were made by Pearson's Chi-square tests or Fisher's exact test. The result of a test was significant if P<5%. Results: The prevalence of HPV was 78.95% with 72.18% HPV-HR of which the most dominant genotype was HPV 56 (46.62%). Prevalence of HPV-LR was 57.14% with a predominance of HPV 42 (31.8%). In our group, 62.48% of women had multiple infections (at least two genotypes). Types 16 and 18 had a prevalence of 20.30% and 8.27% respectively. The rate of HPV infection is high in WLHIV with varied genotype representation. HPV infection was associated with low CD4 count and high viral load (P<0.05). HPV infection was not related to marital status, age, HIV type, treatment, contraception, abortion, nor the number of births (P>0.05). Conclusion: HIV+ women experienced high prevalence of HPV of various genotypes with HPV56, HPV16, HPV82, HPV51 and HPV33 predominant among high-risk genotype and HPV42 and HPV43 in low-risk genotype. Immunosuppression and elevated HIV viral load promotes HPV infection. Correct management of these high-risk population with special care to those who are infected by a genotype not included in the vaccines would p