DOI: 10.36347/sjams.2019.v07i03.002

Pages: 863-870

Acute organophosphorus poisoning is one of the medical emergencies in India. OPCs inhibit Cholinesterase enzyme and cause neuromuscular toxicity whereas OPCs are degraded by a polymorphic enzyme Paraoxonase1 (PON1). Hence PON1 phenotyping in OPC poisoned patients influences their management protocol. Phenotyping can be done by measuring the individual activity of Paraoxonase or Arylesterase or by taking the ratio of their activities. In the present study, we aimed to analyze these methods to assess their reliability by correlating them with cholinesterase activity which is needed for the effective usage of PON1 phenotyping in acute OPC poisoning. Serum Cholinesterase activity was estimated by the DGKC-kinetic method. Arylesterase and Paraoxonase activities of PON1 were estimated by Modified Zeller and Eckerson., et al methods respectively. SPSS software was used for statistical analysis. From this study, it was found that either the paraoxonase activity or the ratio can be used for PON1 phenotyping or to assess PON1 activity in patients with acute OPC poisoning since they had the correlation value of. +0.767 and +0.955. However, it can be concluded that the ‘ratio calculation’ which includes both the allozyme activities of paraoxonase1 is the more reliable method.