DOI: 10.36347/sjams.2019.v07i03.023

Pages: 970-972

Cystic echinococcosis (CE) is a cosmopolitan disease caused by the dog tapeworm Echinococcus granulosus. The disease is difficult to diagnose, treat, and control and is responsible for considerable human morbidity and mortality globally. This study was conducted in Tambool area, and was carried out on cysts collected from infected camels in Tambool market. Cysts were divided into fertile cyst (fluid with scoleces) or sterile (clear fluid). Fertility of collected cysts was determined by placing 1 drop of each hyadatid fluid on slide and covered with cover glass. The slide was examined under the light microscope using X10 objective lens. This study was carried out to determine the survival rate of protoscoleces under different constant temperatures 4c, 25c, 37c and 45c. Cysts were examined for fertility, and then incubated at different constant temperatures. The viability of protoscoleces was determined by using eosin stain, which stains only the dead protoscoleces. The examination was done 1 day, 2 days, and 3 days up to 10 days, post incubation. The result showed that the maximum survival rates observed with protoscoleces kept for 1 day at 4c, 25c and 37c were 92%, 78% and 70% respectively. Since the beginning, no survival was observed in those kept at 45c. In those kept at 25c and 37c, the viability was 0% at the 5th day and 3rd day respectively. With 4c, the protoscoleces remained viable with varying rates till it reached 8% on day 9 and 0% on day 10.