DOI: 10.36347/sjams.2019.v07i03.032

Pages: 1024-1028

Haemoglobin, the red blood cell molecule that carries oxygen, is a vital element of circulatory system. Disorders of haemoglobin include Thalassemia syndromes, structural haemoglobin variants, anemia and polycythemia. Various laboratory methods are proposed for haemoglobin estimation. In our study, we compare automated analyzer and the standard Drabkin’s method for haemoglobin estimation with 1000 samples that were collected randomly from the inpatient and outpatient departments. Results: The mean Hb concentration in Drabkin’s method and automated analyzer in this study are 11.37 g/dl and 11.24 g/dl respectively. Automated method had given the hemoglobin values which are close to the gold standard Drabkin’s method. Comparison analysis results of haemoglobin estimated by Drabkin’s and Automated method showed non-significant difference in hemoglobin estimation. Correlation coefficient showed a maximum significance between Drabkin’s and Automated method (r = 0.98). Conclusion: In this cross- sectional study of haemoglobin estimation we found that the automated analyzer method of haemoglobin estimation is a reliable, time saving and has a minimal manual workload with a better correlation of haemoglobin values with that Drabkin’s method.