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Scholars Academic Journal of Pharmacy | Volume-2 | Issue-04
Development and Validation of Visible Spectrophotometric Method for the Estimation of Lamivudine in Bulk and Pharmaceutical Dosage Form
Sreenivasulu Y, Khadeer Zubair S, Pushpa Latha E
Published: April 30, 2013 | 191 107
DOI: 10.36347/sajp
Pages: 323-326
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Abstract
A Simple, accurate, precise and economical procedure was developed for UV Spectroscopic estimation of Lamivudine in pure state and in its pharmaceutical formulation. The developed method is based upon complex formation of the drug with methyl red reagent. Linearity was observed in the concentration range of 0.5-5 μg/ml. Calibration curve was constructed by plotting absorbance values against concentrations, which gave good regression values. The method was validated statistically and recovery study was performed to confirm the accuracy of the method