DOI: 10.36347/sjet.2019.v07i12.003

Pages: 338-343

Staphylococcus aureus (S．aureus) is an important pathogen causing the mastitis of dairy cows, which often causes great economic losses. In order to prevent the mastitis of dairy cows caused by S．aureus, a successful recombinant plasmid pET-32a-ClfB (ClfB, Clumping factor B), pET-32a-Lbp (lbp, lamin binding protein), and pET- 32 a-ClfB'/ Lbp were transformed into Rosetta (DE3), induced expression and purified. The immunogen was prepared by mixing purified protein and adjuvant to immunize mice and challenge the virus, and the protective difference between single protein immunization and chimerism protein immunization was compared. The results showed that we successfully expressed and purified the protein. After immunizing mice, chimeric proteins were immunized with a single protein, which greatly improved the protective efficiency. This study will lay a foundation for further study of effective vaccines to prevent S.aureus infection.