DOI: 10.36347/sjams.2019.v07i02.057

Pages: 711-714

Alcohol is the main cause of liver injury. Increase in alcohol consumption in society has resulted in an increase in the number of cases of alcoholic liver disease and liver cirrhosis. Methods: This study was performed in the Department of Biochemistry, SVS Institute of Medical Sciences, Mehboobnagar. Based on the inclusion criteria n=20 patients were selected for the study during the study period. Another group of n=20 patients age and sex-matched were selected randomly to act controls. About 5ml of venous blood will be collected under aseptic precautions in a vacutainer and following assays will be done in serum. The serum copper was estimated by the colorimetric method. Serum ceruloplasmin was estimated by the Copper Oxidase Activity Method. Serum tocopherol can measure by their reduction of ferric to ferrous ions which then form a red color complex with α, α-dipyridyl Tocopherols and carotenes are first extracted into xylene and the absorbance is read at 460nm to measure the carotenes. A correction for the carotenes is made after ferric chloride and reading at 520nm. The serum Iron and total iron binding capacity [TIBC] was estimated by the Ferrozine Method. Results: The means and S.D of serum copper levels in controls is 87 ± 20.9 as compared to alcoholic liver disease 51.4±8.1. The means and S.D of serum ceruloplasmin level in control is 45.5 ± 18.5 as compared to alcoholic liver disease 7.8 ± 3.5. The means and S.D of Vit E in controls is 1.1± 0.4 as compared to alcoholic liver disease 0.8 ± 0.2. The means and S.D of serum Iron in controls is 111.2 ± 22.4 as compared to alcoholic liver disease 38.5± 15.3. The means and S.D of serum TIBC levels in control is 304.4 ± 63.1 as compared to alcoholic liver disease is 186.8± 63.3. Conclusion: Therefore it can be concluded from the above study that estimation of copper, ceruloplasmin, vitamin E, Iron, TIBC may receive as a predictive guide and understanding the pathogenesis, the intervention may be initiated at an ea