DOI: 10.36347/sjams.2019.v07i02.077

Pages: 787-791

Introduction: Coronary Heart Disease (CHD) is the leading cause of mortality and morbidity worldwide. Tumour Necrosis Factor type 2(TNFR2) is increased in patients with Coronary Heart Disease and Peripheral Vascular Disease. So genetic polymorphism in TNFR2 may indicate susceptibility to CHD. Aims & objectives: The study aim at whether TNFR2 gene polymorphism is the cause for differences in the Coronary Heart Disease susceptibility among the population. Materials &methods: The study sample comprised of 158 cases of Coronary Heart Disease and 149 age and sex matched controls. Patients were chosen from the Department of Cardiology, Stanley Medical College and Hospital. Subjects with diabetes, hypertension, hypothyroidism and autoimmune diseases were excluded from the control group. 5ml of peripheral venous blood was withdrawn for DNA extraction and lipid profile. 242 bp fragment of TNFR2gene was amplified, followed by restriction digestion.Lipid profile was done by chemical method. Results & observation: The percentage of the MM genotypes of TNFR2 gene polymorphism in patients with Coronary Heart Disease was found to be 39% and 21%in controls ( p value = 0.00) ( odd’s ratio 2.46).RM genotype was found in 79% controls and 57% cases(p value 0.00),(Odd’s ratio 0.36) Among the three genotypes, MM homozygote showed an increased risk. Conclusion: We conclude that MM genotype is an independent risk factor for Coronary Heart Disease.