DOI: 10.36347/sajb.2015.v03i05.010

Pages: 474-478

Infection caused by dermatophytic fungi in man and animals is common throughout the world. Dermatophytoses poses a serious concern to the economically poor population of India. Fungi cause both superficial and internal mycoses. The mycoses, though normally not lethal, is unpleasant and difficult to cure and cause considerable economic loss. Majority of superficial infections are caused by a closely related group of keratinophilic fungi called dermatophytes, which cause ringworm infection or tinea infection. Present study is aimed to develop an antibiotic (Streptomyces species) against dermatophytic fungi. A total of 25 soil samples were used for the isolation of soil microorganisms. They were subjected to primary screening by cross streak plate assay method against dermatophytic fungi. Then they were subjected to secondary screening by cross streak plate assay method to further test the capabilities of primarily screened organisms. Finally 5 isolates were selected for further study on the basis of (a) broad spectrum activity and (b) larger zone of inhibition in comparison to others. The antimicrobial substances were extracted with ethyl acetate from isolate- inoculated Kuster’s broth fermented for 7 days at 28ºC by solvent extraction method. Minimum inhibitory concentration (MIC) was determined.