DOI: 10.36347/sajb.2015.v03i07.005

Pages: 603-606

The study was aimed to compare the relative yield, purity and integrity of RNA extracted from leaf tissues of different plants using the modified CTAB method developed by Chang et al. 1993. Extraction of RNA is very crucial step in the gene expression analysis of plants. In this study, a large number of samples such as fresh leaves obtained from different plant species Oryza sativa, Elusine korakkana, Zea mays, Azadirachta indica, Musa sapianatum, Sesbania grandiflora were taken and modified CTAB method was applied for RNA extraction. Quality of the extracted RNA was tested by measuring the absorbance of RNA at 260 nm using Nanodrop® ND-1000 spectrophotometer and measuring the ratio of A260 / A280 respectively. Amplifiable quality of RNA with satisfactory concentration were tested by running a horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V confirms that the modified CTAB method could be recommended for the all the tested plant species except Musa sapianatum.