DOI: 10.36347/sajb.2015.v03i10.001

Pages: 828-832

The present study explained about the effect of chlorpyrifos pesticide on rhizospheric soil from the plant of Calotrophis gigatica has been investigated. The current investigation was carried out the objectives such as biodegradation of chlorpyrifos by Staphylococcus sp. isolated from the soil previously exposed to chlorpyrifos. Subsequently to study the toxicity of different bioremediated media using L. reticulates as a test animal after different days of incubation. Staphylococcus species is a native soil bacterium growing on the cholrpyrifos mixed in the broth medium. To investigate whether the chlorpyrifos has been degraded by the bacteria both control (broth + chlorpyrifos) and experimental medium (broth + chlorpyrifos + Staphylococcus bacteria) were exposed to L. reticultes after 24, 48, 72 and 96hrs of incubation. When the experimental fish L. reticulates fed with control broth medium there was a heavy mortality within the short period of time in 12ppm concentration. While the fishes survived when fed with broth containing chlorpyrifos incubated with bacterium for 96hrs. From the present study confirmed that the isolated chlorpyrifos degrading bacterium could be used successfully for the removal of the pesticides in agriculture their use and toxicity it is recommended that research or biodegradation particularly on bioremediation sites particularly pesticides contaminated areas in agricultural field.