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Scholars Academic Journal of Biosciences | Volume-3 | Issue-10
Cellulase-assisted extraction of pleurotus eryngii polysaccharide
Qin Li, Shaoying Zhang , Lifen Hu
Published: Oct. 31, 2015 | 61 54
DOI: 10.36347/sajb.2015.v03i10.007
Pages: 856-859
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Abstract
The pleurotus eryngii polysaccharide was extracted through using cellulase as subsidiary condition. The optimized conditions were studied by one-factor experiment and orthogonal experiment. The result showed that the influential degree weakened as follows sequence: pH, enzyme quantity, time and temperature. The suitable condition for extraction of pleurotus eryngii polysaccharide was enzyme quantity 0.030%, pH 4.5, temperature 55℃, and 40 minutes with 5 g of pleurotus eryngii in 100 mL enzyme suspension. Under optimized condition, the pleurotus eryngii polysaccharide yield was 27.0 mg/Kg.