DOI: 10.36347/sajb.2016.v04i01.002

Pages: 4-10

Amylase is known for the breakdown of glycosidic linkages present in starch into low molecular weight products such as glucose, maltose, maltotriose units, limit dextrins. Due to this property of amylase has been exploited in textile and detergent industries for desizing and stain vanishing. Upon immobilization, its thermal and storage stability was also found to be increased. It was also immobilized onto bovine serum albumin (BSA) to prepare its BSA bound nanoparticles. In present study, extracted amylase of Pearl millet seedlings was immobilized in BSA nanoparticles with four different emulsifier namely, Almond Oil, Jasmine Oil, Mustard Oil, and Olive Oil. These nanoparticles were used as bio-active detergent laundry additives with 40U of alkaline protease and different detergents; Ariel matic, Surf excel liquid, Active wheel and Tide plus to test their stain removal competency. In this study, Chosen strains were comprised of cosmetic strains of nail paints of some of popular international brands, namely; Chamber Geneva, Revlon, Deborah Milano and Givenchy. These cosmetics stains are tough to wash off from clothes and often require pre-washing practices. Hence, from this study, an effective washing analysis was carried out and strains are found to be very well wash off if when washed with chosen detergents with bio-active prepared amylase bound nanoparticles solution as compared to when washing was done with chosen detergents only. Among them, Ariel matic detergent with enzyme loaded bovine serum albumin nanoparticles was found to be have best washing results as bio-active system compared to other chosen detergent samples, followed by Surf excel liquid, Tide plus and the least effective was found to be Active wheel.