DOI: 10.36347/sajb.2016.v04i02.008

Pages: 123-127

This study was conducted to evaluate the bacterial load of water from the pump well used by the residents of Linao East and West in Tuguegarao City for the reason that the Barangay is located in a low lying area, that made them prone to flood. The researchers gathered the water samples from all the pump wells in Barangay Linao East and West Tuguegarao City and used Experimental Design to assess the potability of aforesaid water resources. Samples were collected by using sterilized bottles as a container prior to collection the mouth of the pump well was sterilized. It was then inoculated in the Lauryl Tryptose Broth as a presumptive test for the identification of Coliform bacteria after 24-48 hrs. incubation period followed by the Brilliant Green Lactose Bile Broth as a confirmatory test after 24 hrs. incubation period and finally the EC Broth as a completion test to identify the presence of E. coli after 24 hrs. of incubation. Production of gas bubbles will be observed as an indication of coliform bacteria. These were conducted at the Metropolitan Water District, Tuguegarao City. Based on the results of the Multiple Tube Fermentation Tests done, it showed that there are water samples positive of Escherichia coli at Barangay Linao East and Linao West. There are 29 pump wells in Barangay Linao East and 15 pump wells for Barangay Linao West are considered not potable as they contain coliform bacteria that can bring about gastroenteritis. The possible reason for this outcome is the Geographical location of the both Barangays which is situated in a low lying area and they too have poor environmental hygiene such as poor solid waste disposal and excreta disposal that may lead to water contamination. Aside from this, pump wells are not being examined and treated against pathogenic microorganisms like chlorination, also boiling of water from the pump well is not being performed. Coliform bacteria - a rod-shaped microorganism that will be tested in this study and which in