DOI: 10.36347/sjams.2016.v04i11.008

Pages: 3896-3899

Tissue microarray is very useful in research. However the automated equipment is very costly and cannot be afforded by many laboratories. We describe a very simple and cheap manual method of preparation of tissue microarray using readily available materials. Materials and methods: tissue cores were punched out from the donor blocks using a 16 g bone marrow trephine biopsy needle. The cores were aligned in a matrix of 6x5 on a transparent adhesive. L moulds were places around the matrix and molten paraffin wax was poured to form a paraffin block. Results: The block was then cut with a microtome and sections were used for immunohistochemistry and H & E. Excellent results were obtained from the method. Discussion: We have described a very simple and cheap method of construction of tissue microarray by using readily available materials, which does not require any special training or expertise. The method can be followed in any histopathology laboratory and its uses are many in the field of research, standardisation of antibodies and in situ hybridization.