DOI: 10.36347/sajp

Pages: 74-80

Traditional ethnomedicinal use of plant is recognized as an important potential source for compounds used in mainstream medicine. Herein, the in-vitro antioxidant property and phytochemical composition of Pergularia tomentosa L. plants widely used was evaluated. 1, 1-diphenyl-2-2-picrylhydryzyl (DPPH) radical scavenging effect of the crude extract and its fractions were determined between 0.1, 0.25, 0.5, 1.0 2.5, and 5.0mg/ml spectrophotometrically. While, qualitative phytochemical test revealed the presence of phenolics, flavonoids alkaloids, saponins, steroids and glycoside. A systemic activity trends analysis indicated that P. tomentosa L. whole plant has relative antioxidant activity ranged from crudes extract to basic, acidic, methanolic and hexane fractions in the order of increasing sequence (CRD>BSC>ACD>MET>HEX). The crude extract haven highest antioxidant activity, followed by the basic fraction (alkaloid) respectively compared to acidic and methanolic fraction. Hexane has the lowest activity. This studied medicinal plant have interesting antioxidant properties and a phytochemical composition that could provide scientific evidence for some folk uses in the treatment of diseases related to the production of ROS and oxidative stress. Thus, trends for activity flow of antioxidant properties demonstrated in this work will provide evidence for researcher to isolate active antioxidant compound from the fraction with highest activity (basic fraction i.e. alkaloid)