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Scholars Academic Journal of Pharmacy | Volume-4 | Issue-02
Antifilarial activity of Methanolic extract of Vitex negundo L. leaves against Setaria cervi filarial parasite.
Keertisheel N Sahare, Vinod Singh
Published: Feb. 28, 2015 |
99
81
DOI: 10.36347/sajp
Pages: 88-92
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Abstract
Herbal medicinal practice is well known but largely observed all over the world. Currently available
antifilarial drugs Diethylcarbamazine citrate, albandazole, Ivermectin and other combination of drugs are not effective
against adult parasite. So adulticidal antifilarial drugs is required to control and management of the disease. In the present
investigation, antifilarial activity was assessed for Methanolic extract of Vitex negundo L. Leaves against Setaria cervi
filarial parasite. Activity was assessed by the method of motility inhibition and MTT reduction assay with concentrations
0.3-0.006 mgmL-1 for 2 to 24 hrs incubation period respectively, by comparing with control. In motility assay, complete
inhibition of motility was observed and in MTT reduction assay which gave >50% reduction for concentrations 0.06, 0.1
and 0.3 mgmL-1 at 10, 6 and 2hrs incubation period respectively in a dose dependent manner (p<0.05). Antifilarial
activity given by plant extract was found to be a function at their relative concentrations. Inhibitory concentration (IC50)
for the plant extract was found to be 0.049mgmL-1. Vitex negundo L. Leaves Methanolic extract showed significant
reduction in adult motility in dose dependent manner contributes to the development of database and isolation of active
molecule / principle for novel antifilarial drug candidate