DOI: 10.36347/sjams.2019.v07i12.066

Pages: 4162-4167

Background: The imbalance between pro- and antioxidant mechanisms favoring oxidative stress is known to be involved in pathogenesis of cancer. Antineoplastic agents used in treatment of metastatic breast cancer acts by generating reactive free radicals. Reactive radicals produced due to metastatic breast disease and metabolism of chemotherapy drugs are detoxified mainly through glutathione antioxidant system. Objectives: To evaluate differential serum glutathione peroxidase and glutathione-s-transferase activity and serum reduced glutathione concentrations among patients with metastatic breast disease receiving chemotherapy with different drugs. Material and Methods: We selected thirty metastatic female breast cancer patients and blood samples were collected before and after first chemotherapy cycle. Thirty healthy female volunteers from similar age group were selected as controls. Serum glutathione peroxidase activity was determined by ELISA. Serum glutathione-s-transferase activity and reduced glutathione concentration was measured by spectrophotometric methods. Results: Glutathione peroxidase and glutathione-s-transferase activity was significantly higher and serum reduced glutathione concentration was significantly lower in metastatic breast disease before receiving chemotherapy compare to healthy controls (P<0.0001). After chemotherapy, glutathione peroxidase (P=0.022), glutathione-s-transferase (P<0.0001) activity as well as reduced glutathione (P<0.0001) concentration were significantly decreased as compare to their concentration before chemotherapy. No significant difference was observed in the levels of these antioxidants among different chemotherapy groups. Conclusion: These findings provide an insight on systemic redox state and differential antioxidant status in metastatic breast disease among different chemotherapy treated groups. Evaluation of these antioxidants may be useful in chemotherapy treatment monitoring and prognosis of metastatic breast .....