DOI: 10.21276/sajp.2016.5.9.1

Pages: 363-370

The present study is an effort to demonstrate the role of Chrysin in the management of Collagenase-ICV induced brain stroke in rats. Wistar rats either sex 200-250 g. Animals were divided into 6 groups (n=6). Chrysin was administered daily in 3 doses (10, 20 and 40 mg/kg; p.o.) to rats for 28 successive days. Brain stroke was induced in rats by intracerebroventricular (ICV) injection of collagenase (10 µl/ml) using stereotaxic appratus. Behavioural assessment of rats was carried out with the help of Elevated plus Maze, Novel objective recognition and locomotor activity were carried out. After behavioural evaluation, the animals were sacrificed and their brains were isolated for estimating brain TBARS, GSH, Nitrite, Catalase and protein levels. Brain stroke (induced by collageanse, 10 µl/ml) significantly increased the transfer latency (TL) of rats in elevated plus maze studies and showed significant difference in exploration of the novel and familiar object in retention trial of rat in novel objective recognition task as compared to sham control group. Collgenase treated rats showed significant decrease in brain GSH, catalase, protein levels and significant increase in brain TBARS and nitrite levels as compared to sham control group. Administration of Chrysin (10, 20 and 40 mg/kg; p.o) significant (p<0.05) reduced the TL of rats during elevated plus maze session, significant increases the time spent exploring the novel compared familiar object as compared to sham control group. Chrysin treated rats showed (p<0.05) an increase in GSH, catalase, protein levels and (p<0.05) decrease in brain TBARS and nitrite levels. Thus, Chrysin may prove to be a useful remedy for the management of brain stroke owing to its possible neuroprotective and antioxidant properties.