DOI: 10.21276/sajp.2017.6.4

Pages: 27-33

A simple, Accurate, precise method was developed for the simultaneous estimation of the Miconazole and Clindamycin in Tablet dosage form by RP-HPLC. Chromatogram was run through kromasil 150mm x 4.6 mm, 5. Mobile phase containing Buffer and Acetonitrile taken in the ratio 20:80 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.01N KH2PO4solution. Temperature was maintained at 30°C. Optimized wavelength for Miconazole and Clindamycin was 210nm. Retention time of Miconazole and Clindamycin were found to be 2.076min and 2.795min. % RSD of the Miconazole and Clindamycin were and found to be 0.99 and 1.05 respectively. Percentage assay was obtained as 100.23% and 99.25% for Miconazole and Clindamycin respectively. LOD, LOQ values are obtained from regression equations of Miconazole and Clindamycin were 1.42ppm, 0.60ppm and 4.31pm, 1.82ppm respectively. Regression equation of Miconazole is y = 19688x + 25044, and y = 18253x + 2735.5 of Clindamycin.