DOI: 10.21276/sajp

Pages: 120-125

The present study was aimed to Isolate and characterize the active constituents from higher Basidiomycetes Calocybe Indica Apk-2, an indigenious variety developed by Tamil Nadu Agricultural University, Coimbatore. Extraction was done with various solvents like petroleum ether, ethyl acetate, chloroform, methanol and water. Methanolic extract was found to contain Alkaloids, Carbohydrates, Glycosides, Phytosterols, Terpenoids and Flavonoids. Aqueous extract was found to contain Protein, Amino acids, Carbohydrates and Glycosides. Methanolic extract was found to have significant inhibitory activity against Bacillus cereus and Bacillus lentus and moderate effect against other micro organisms. Aqueous extract was found to have significant activity against pseudomonas and azotogensis and bacillus cereus. Methanolic extract was subjected to column chromatography to isolate its components and the active compound was characterised by using spectral analysis.