DOI: 10.36347/sjams.2018.v06i12.059

Pages: 4964-4976

Pseudomonas is a group of bacteria, which is widely distributed in soil, water, skin flora and most man- made environments throughout the world. Due to multidrug resistance patterns of Pseudomonas, it is imperative to know the institutional prevalent susceptibility profiles. This study was conducted to isolate different species of Pseudomonas from various clinical samples, to determine the antibiotic susceptibility pattern and to carry out the epidemiological investigation of the isolates. The study was conducted in a tertiary care hospital, over a period of 2 years. After identification of genus Pseudomonas, the speciation was done by biochemical tests and by VITEK 2. Antibiotic susceptibility was determined by disc diffusion method. Extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) production were detected by the combined disc diffusion test. An epidemiological study of Pseudomonaswas carried out. Of 5096 infected samples, 505 (9.9%) were non-fermenting Gram-negative bacilli, among which 1 were found to be Acinetobacter.