DOI: 10.36347/sjams.2018.v06i12.067

Pages: 5014-5026

A Dot enzyme linked immonosrbent assay (Dot ELISA) was developed and compared with standard indiect plate ELISA and gas chromatography (GC) for chlorpyrifos detection in the field samples. Firstly Dot ELISA for detection of Antichlorpyrifos antibodies (ACA) with prepared antigen and pure chlorpyrifos was standardized with the dilution range of 1µg and 1:1000µg/ml considering its limit of detection (LOD) value. Further since Dot ELISA was evaluated for field samples (fruit fodder, vegetables) for which, known dose of pure chlorpyrifos were spiked into field samples and determined with indirect plate ELISA, Dot ELISA and compared with GC. During the study four other parameters viz. sensitivity, specificity, accuracy, and K value were also determined by using Kappa statatics. Kappa statatics was used to interpret the comparative result between both types of ELISA. Results obtained from the studies showed accuracy between (50 -60%) sensitivity (10-70%) and specificity (12-90%).