DOI: 10.36347/sjams.2017.v05i06.002

Pages: 2041-2046

Flow cytometry is highly sensitive for detection and quantitative analysis of surface and intracellular antigens in malignant hematopoietic cells. Immunophenotyping of leukemia cells play crucial role in identification of leukemia cell line, which in turn serves for individual treatment monitoring and detection of residual disease. The main purpose of this study is to identify the expression and frequency of various cytoplasmic and surface markers in acute monoblastic leukemia. We have included 30 cases of acute monoblastic leukemia with applied primary and secondary panels of markers for immunophenotyping. We analysed frequency and expression of different subsets with combination of positive and negative markers. CD15 and CD11c were found to be positive in all cases while expression of CD14 was found to be variable. CD19 was aberrantly expressed in 13.3% cases. Our study indicates that all 30 cases were negative for B cell and T cell markers. Amongst the secondary panel for monocytic markers CD15 and CD11c were found to be highly sensitive and specific while CD14 was variable in its expression.