DOI: 10.21276/sajp.2018.7.2.1

Pages: 60-66

Ischemic bowel disease is induced by poor circulation of blood, and thus oxygen and nutrition necessary for the small intestine are not supplied enough. As a result, such condition causes inflammation and ulcers, such as ischemic colitis and acute mesenteric arterial occlusion. We previously reported changes in function and expression level of P-glycoprotein (P-gp) which is a typical and important ABC transporter in the small intestine, liver and kidney during intestinal ischemia/reperfusion (I/R). In this study, we examined I/R-induced changes in multidrug resistance-associated proteins (Mrps) which is also an important ABC transporter like P-gp. The superior mesenteric vein and artery in rat were occluded by hanging itself using surgical-sutures connected with the spring balance for 60 min, followed by a reperfusion. The excretion clearances to jejunal lumen (CLjejunum), bile (CLbile) and urine (CLurine) of 5(6)-carboxyfluorescein (5-CF) diacetate (5-CFDA),