DOI: 10.21276/sajp.2018.7.4.3

Pages: 189-193

Magnetic solid lipid nanoparticles (MSLNs) made of iron oxide cores embedded within a glyceryltrimyristate solid matrix have demonstrated hem compatibility, appropriate magnetic response and adequate heating characteristics to be used in hyperthermia. We now tested the association of MSLNs to 5-fluorouracil (5-FU), a drug of chose in colon cancer treatment, to determine the ant proliferative effect modulation. We used HCT15 resistant and T-84colon cancer cells. The non-magnetic solid lipid nanoparticles (SLNs) were included as a control. In addition, we analyze the effect of both MSLNs and SLN in relation to the P-glycoprotein activity, a resistance mechanism implicated in colon cancer chemotherapy failure. Our results showed that both MSLNP and SLNP did not induce cell toxicity and that both NPs loading 5-FU (SLNP-5-FU and MSLNP-5-FU) did not modulate free 5-FU activity against T-84 and HCT15 cells. In addition, fluorescent microscopy analysis showed that none of the NPs was able to decrease rhodamine 123 into tumor cells indicating absence of activity in relation to P-glycoprotein pump.