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Scholars Academic Journal of Pharmacy | Volume-7 | Issue-08
The Antibiotic and Garlic Susceptibility of Escherichia coli Strains and Their Ability of Attachment and Biofilm Formation
Ameyapoh, Nakpane Fankibe
Published: Aug. 30, 2018 |
241
100
DOI: 10.21276/sajp.2018.7.8.2
Pages: 365-371
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Abstract
This study consisted on the impact of antimicrobial agents on Escherichia coli
(E. coli) isolates and their power to adhere to surface for biofilms formation. Sixty two E.
coli isolated from patients urines were then used for standard antibiograms on Mueller
Hinton (MH) agar associated with garlic juice antimicrobial activity. The attachment of
three E. coli strains selected according to their susceptibility to antibiotics was
experimentally performed on glass slides and the adhesion profiles were determined to
assess their power of biofilm formation. As results, even though, the dominant types of
resistance were respectively to amoxicillin, tetracycline, ampicillin and nalidixic acid in
more than 80% of all the E. coli isolates, the highest sensitivity were observed with
Gentamycin in 81% and imipenem in 97% of all the samples. In addition, the tests of
adhesion revealed that, significantly (p <0.0001), the three E. coli attachment to surface
increased with their resistance to antibiotics. However, none of them resisted the garlic
juice as well as isogenic planctonic-growing bacteria as biofilm-ones.