DOI: 10.36347/sajb.2023.v11i05.001

Pages: 163-170

Plants are emerging as a vital source of microbial diversity and, therefore, of bioactive secondary metabolites. This study aimed to metabolomes profiling and invistigate the antimicrobial activity of actinobacteria associated with plants collected from thumama desert in Riyadh region, Saudi Arabia. Assessment of the antimicrobial potential has been done using agar well diffusion method and the metabolites profiling has been carried out using HPLC and GC-MS. Several actinobacterial extracts showed antimicrobial activity against multiple tested microbes, NA118 and N182 exhibited antimicrobial activity against the three tested microbes Klebsiella pneumoniae, Bacillus subtilis and Candida albicans. Actinobacterial strains showed differential levels of amino acids, Isolate NA118 showed the highest increases in valine, proline, ornithine, leucine. Highest levels of hexadecatrienoic (C16:3) and hexadecanoic (C16:0) were recorded for isolate NA8. Isolate NA182 was rich in several phenolics and flavonoids. High contents of beta-tocopherol and delta-tocopherol and gama-tocopherol were recorded for isolate NA118 and NA18. Finally, The pharmacological characteristics of the bacterial extracts were assessed. Antibacterial testing revealed that 10 of 12 bacterial extracts were having antimicrobial activities against at least one of the microorganisms examined. Therefore, Further studies needs to be performed in which we can isolate and identify various actinobacterial species using different media beside it could have interesting biologically active metabolites.