DOI: 10.21276/sajp.2019.8.8.8

Pages: 433-435

RP-HPLC is fast, simple, sensitive, precise, and reproducible (liquid chromatography) method was developed and validated for the analysis of vandetanib bulk dosage form. Using C-18 HPLC column separation was carried out. Which was maintained at ambient temperature. During separation mobile phase consist of methanol (100 v/v) was delivered at a rate of 1mL/min. Analysis was carried out by using UV detector at the wavelength 328 nm. In RP-HPLC method was validated by using various parameters like, precision, limit of quantitation (LOQ), linearity and robustness. The RP-HPLC method was found to be linear over the concentration ranges from 50-100 μg/mL (r2 =0.9996). Retention time for bulk vandetanib was found to be 5.496±25 min. LOQ of method was 6.8339 μg/mL and LOD 2.7036μg/mL. Thus, the RP-HPLC developed method was found to be robust and rugged which can be applied for the regular analysis of vandetanib in the bulk as well as pharmaceutical dosage form.