DOI: 10.36347/sjebm.2019.v06i07.002

Pages: 345-350

In this study, strains with transforming ginsenoside activity were screened from fresh ginseng rhizosphere soil in changbai mountain, huai hua, ji 'an and yulin town, tonghua city, jilin province, China.Single colony wasscreened by tablet marking method with ginseng stem leaf total ginsenoside as substrates for the liquid fermentation. Chromatography and thin-layer chromatography were used for preliminarily determine conversion activity, ultraviolet spectrophotometry determined the ginseng total ginsenoside of conversion rate. PCR and DNA sequencing technology were for identify the best strain of active species. Finally, 16 strains were isolated from the rhizosphere soil of fresh ginseng, among which the code C4 strain had the best transformation activity, which increased the content of ginseng stem and leaf ginsenoside by 28.7%. The phylogenetic tree was constructed by neighbor-joining method, it belongs to Trichoderma.