DOI: 10.36347/sajb.2016.v04i10.020

Pages: 911-916

A simple and inexpensive method was developed using solid-phase extraction, together with gas chromatographic method for determination of difenoconazole residues in pomegranate fruits. The evaluated parameters include the extracts by deactivated acidic alumina chromatographic column using hexane + di ethyl ether (9:1 and 8:2, v/v) mixture, hexane and acetonitrile solvents. The method was validated using pomegranate fruit samples spiked with difenoconazole at different fortification levels (0.01 and 0.1 µg/g). Average recoveries (using each concentration six replicates) ranged 83-95%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.005-1.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.005µg/g and 0.01µg/g respectively. Finally the pomegranate fruit residue samples were analyzed by GC.