DOI: 10.36347/sajb.2016.v04i11.009

Pages: 1022-1031

The biosynthesis of metal nanoparticles has become a matter of great interest in recent times due to their various advantageous properties and applications in a variety of fields. The exploitation of different fungi for the biosynthesis of nanoparticles is considered a green and eco-friendly technology. In this study, Silver, Copper, Zinc and Manganese nanoparticles were synthesized using a rapid and completely green biosynthetic method by reduction of AgNO3, CuSO4, ZnSO4 and MnSO4 solution with cell free filtrate of four different funguses viz. Alternaria alternata, Fusarium brachygibbosum, Penicillium verruculosum and Aspergillus niger were carried out. The biosynthesis of metal nanoparticles was confirmed by metal ion reduction test. In the UV-Visible spectroscopy the maximum absorption peak of the ZnNP, AgNP, MnNP and CuNP was found at 360 nm, 420 nm, 425 nm and 610 nm respectively. The structural and properties of the metal nanoparticles were investigated by X-ray diffraction, Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The average particle size as determined by XRD was found to be 2-3 nm in case of Silver, Copper and Zinc whereas 24 nm in case of Manganese nanoparticles with crystalline in nature. SEM analysis showed the spherical shape of nanoparticles. The nanoparticles synthesized through this biosynthesis method can potentially useful in various applications.