DOI: 10.36347/sajb.2016.v04i11.013

Pages: 1054-1059

Multi drug resistance Klebsiella pneumoniaee are rapidly emerging as life threatening nosocomial infections. The ICUs are considered as epicenter of infection mainly because of severe clinical conditions, increasing use of invasive diagnostic procedures lapses in sterilization and disinfections. Βeta-lactamase which include ESBL, AMPC, CARBAPENAMASES, MBL have emerged as the most worrisome mechanism of resistance amongst the gram negative bacteria which pose a therapeutic challenge to health care institutes. This study aims to find the prevalence of MDR Klebsiella pneumoniaee isolates from various clinical specimens of ICU patients and to evaluate their sensitivity patterns. The present study was a retrospective study of a 50 bedded ICU in tertiary care hospital from August 2014 to May 2015. Out of total 500 different clinical specimens studied 288 (57.6 %) specimens showed growth of Klebsiella species. Phenotypic characterizations of Beta lactamases present in Klebsiella isolates were carried out by disc diffusion methods (CLSI guidelines). The prevalence of Beta lactamase genes in 25 strains of Klebsiella was also studied using multiplex PCR. Antibiotic susceptibility was performed according to the CLSI guidelines. Among the total of 500 isolates from ICU 288 (57.6 %) isolates were Klebsiella maximum being isolated from tracheal specimens. The most common βlactamase identified was AMPC 151 (52.43%), followed by carbapenames 45 (15.62%), ESBL 31 (10.76%), (7.29%), MBL 5 (1.73%), unknown 25 (%), Coproduction of ESBL +AMPC 17(5.90%), AMPC+CARBAPENAMSES 8 (2.77%), AMPC +MBL 4 (1.38%) was detected phenotypically and similar results were found genotypically.