DOI: 10.36347/sjams.2018.v06i01.051

Pages: 247-253

The study was carried out in the Department of Microbiology Index Medical College, Hospital and research centre (IMCHRC) Indore. In 100 diabetic foot patients studied, 73% were male and 27% were female. The age ranged from 31-80 yrs and majority of patients were in the age group o 51- 60 yrs. Out of 100 patients, 53 patients were diagnosed to have diabetes mellitus for> 10 yrs, 35 for 5 - 10 yrs and 12 for < 5 yrs. Out of 100 patients majority of the patients (90.29%) had ulcer for > 1 month duration. Out of 100 patients, 17 patients with ulcer belonged to the Wagner's grade 1, while 31 belonged to Grade 2, 40 belonged to grade 3 and 12 belonged to Grade 4. No growth was seen in 6 specimens out of 100 specimens. From 94 patients with culture positive specimens 170 organisms were isolated. Out of 94 culture positive cases, 22 patients had only 1 isolate, 72 patients had polymicrobial isolates Among the bacterial isolates, Gram negative comprised of 97 (57.1%) and Gram positive accounted for 73 (42.9%). Pseudomonas spp. was the most common isolates, accounting for 23.5% of all isolates followed by Staph. Aureus, Esch. Coli and Enterococci spp. comprising of 19.4%,10.5% and 10% respectively. All strains of S. aureus were sensitive to Vancomycin, 63.6% were sensitive to gentamicin and 60.60% were sensitive to cotrimoxazole. Erythromycin and penicillin showed least sensitivity 36.36% and 36.3% respectively. Enterococci spp. showed 100% sensitivity to vancomycin, 70.59% were sensitive to amikacin and gentamicin. Coagulase Negative Staphylococci showed relatively high sensitivity to all antibiotics, gentamicin 56.5% ciprofloxacin 82.6% penicillin 56.5% erythromycin 52% and cotrimoxazole 52% Among Pseudomonas spp. 90% strains showed sensitivity to Imipenem, 90% were sensitive to Amikacin, 70% were sensitive to Gentamicin. Pipercillin - Tazobactam combination showed sensitivity of 62.5 % Ceftazidime and Piperacillin had least acivity 45% and 30 % respectively. All other Gra