DOI: 10.36347/sjams.2018.v06i01.063

Pages: 317-322

The fixation also in optical differentiation of cells and tissue constituents by altering their refractive indices in varying degrees. The fixation is a critical step in the preparation of histological sections by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. This is of value in the microscopic examination of cells and tissue. The present study aimed to find the best fixative for a particular organ, so that the best histological section details can be produced. We studied the effect of five different types of fixatives. An essential part of all histological and cytological techniques is preservation of cells and tissues as they naturally occur. To accomplish this, tissue blocks, sections or smears are usually immersed in a fixative fluid, although in the case of smears, merely drying the preparation acts as a form of preservation. The aim of the current study is to see the effect of the following fixatives namely 10% formalin, Buffered 10% formalin, Bouin’s fluid, Zenker’s fluid, Carnoy’s fluid on liver tissues and to observe the optimum result in a particular fixative in H&E sections. There is no single fixative which can be considered as best fixative for all purposes. Best fixatives for architectural preservation nare Carnoy’s fluid and Zenker’s fluid. Best fixative for study of nuclear details is Bouin’s fluid.