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Scholars Journal of Applied Medical Sciences | Volume-5 | Issue-08
Multiplex Polymerase Chain Reaction for Detection of 16 Respiratory Viruses
Karima Rissoul, Hakima Kabbaj, Ahmed Essaidi Alaoui, N Ajat Bouihat, MouniaQrafli, Imane Jroundi, Myriam Seffar
Published: Aug. 31, 2015 | 148 141
DOI: 10.36347/sjams.2017.v5i08.016
Pages: 3035-3041
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Abstract
The objective of this study is to identify different respiratory viruses in a population of patients hospitalized at Rabat Hospital for severe acute respiratory infection for a period of one year. Over the period: from 12/09/2013 to 09/12/2014, 62 respiratory samples from 59 patients reached the central laboratory of virology. All samples were extracted using QIAGEN columns and analyzed by multiplex PCR. 67.7% of the samples are positive and 32.3% negative. 78.5% from pediatric and 21.5% from adult intensive care. Co infection viral rate was 48.5% in pediatrics and 33.3% in adult intensive care. Rhinovirus is the most frequently isolated in both pediatric and adult intensive care, it is also the most found in the viral co-infections, the association with bocavirus is the most common. In winter, the number of positive samples was the largest compared to other seasons with predominantly of rhinovirus, adenovirus and respiratory syncytial virus A.