DOI: 10.36347/sjams.2017.v05i10.035

Pages: 3978-3983

Extended-spectrum β-lactamase (ESBL) production is the major resistance mechanism to β-lactam antibiotics in Enterobacteriaceae. In addition, emergence of plasmid-mediated quinolone resistance (PMQR) in ESBL-producing isolates has become a global threat for treatment of these infections. The aim of the study was to analyze ESBL production among Klebsiella species by combined disc diffusion method and to detect fluoroquinolone resistance by Kirby Bauer disc diffusion method along with the MIC by E- strip method. A total of 200 isolates of Klebsiella species were collected from different non repeated clinical samples and Antibiotic susceptibility test with special reference to fluoroquinolone antibiotics was performed by disc diffusion and ESBL production was confirmed by combined disc diffusion method. Among 200 Klebsiella isolates, 121(60.5%) were ESBL producers. Out of these 121 ESBL isolates, Multidrug-resistance was observed in 80.9 % isolates. Fluoroquinolone resistance was detected among ESBL isolates of which; 76% were resistant to nalidixic acid and 76% to ciprofloxacin, 74.4% levofloxacin, 76% to sparfloxacin and 76% to moxifloxacin. For carbapenem antibiotics; 55.4 % and 54.5% ESBL isolates were susceptible to imipenem and meropenem respectively. Resistance to Piperacillin and Piperacillin/tazobactam were 90.1% and 72.7%, respectively. Bacterial resistant profile found to be quite high to ESBL production with quinolone resistance in the majority of the Klebsiella isolates, which are emerging because of prescription of improper antibiogram schedules.