DOI: 10.36347/sjams.2018.v06i02.002

Pages: 457-465

Enterobacteriaceae group is the main cause of bacterial infection; in this family Escherichia coli and Klebsiella spp. are the most prevalent causes of nosocomial infections. Extended-spectrum β-lactamases (ESBLs) represents a major threat among multidrug-resistant bacteria. Metallo-beta-lactamase (MBL) producing gram negative bacteria are being reported from several parts of the world and have emerged and clinically significant carbapenem resistance mechanisms. MBL producing bacteria can hydrolyze a wide range of beta-lactam antibiotics and lack the ability to hydrolyze aztreonam. This study was conducted in indoor department of Teerthanker Mahaveer Medical College & Research Centre, Moradabad, U.P. which is a tertiary care hospital. This study was conducted during the period from Jan 2015 to June 2016. A total of 200 gram negative Enterobacteriaceae were isolated in 500 no. of samples. They were screened for the β-lactamase production. Among the 500 isolates β-lactamase production was observed in 200 strains. 114 (57%) strains were ESBL producers; followed by 60 (30%) strains were MBL producers. The major ESBL and MBL producer was E.coli. Multidrug resistance to the fluoroquinolones and the aminoglycosides was also observed in the β–lactamase producing organism. The high prevalence of β-lactamases in ICU. The ESBL-producing organisms are a breed of multidrug-resistant pathogens that are increasing rapidly and becoming a major problem in the area of infectious diseases.