DOI: 10.36347/sjams.2018.v06i02.003

Pages: 466-472

Pseudomonas aeruginosa is an increasingly prevalent opportunistic human pathogen and the most common gram negative bacterium found in nosocomial infection. Production of metallo-β- lactamase enzyme in Pseudomonas aeruginosa usually results in high level resistance to most β-lactamases and a rapid spread of MBL producing major gram negative pathogens is a matter of particular concern worldwide. To identify the metallo –β-lactmase production by double disk synergy test, combined disk diffusion method and modified hodge test as described further. Out of 514 samples, on the basis of gram staining , 108(21.01%) gram positive cocci, 296(57.58%) gram negative bacilli, 89(17.31%) no organism seen and 21(4.08%) were yeast.On the basis of culture of 89 samples , 11 (12.35%) were gram positive cocci, 23 (25.84%) were gram negative bacilli, 55(61.81%) were showed no growth. In 319 samples of gram negative bacilli, 100 were isolates of Pseudomonas aeruginosa and in which 18 were found to be producing metallo β-lactmases. This study suggests metallo-beta-lactmase production in pseudomonas aeruginosa is emerging threat in hospital isolates because it is crucial for the optimal treatment of patients.