DOI: 10.36347/sjavs.2014.v01i02.003

Pages: 58-63

Simple sequence repeat (SSR) analysis was used to identify the genetic status 23 citrus somatic hybrid genotypes from Siam Madu +Satsuma Mandarin and their similarity to the parental. Five SSR primer pairs could amplify polymorphic SSRs from all of these genotypes. A total of 17 alleles with an average of 3.4 alleles per locus were detected. The polymorphic information content (PIC) among genotypes varied from 0.076 (TC26 285) to 0.673 (AG14). Pairwise coefficients of genetic similarity between all genotypes ranged from 0.24 to 1.00. Unweighted pair-group method arithmetic average (UPGMA) analysis allocated the genotypes and their parents in 4 major clusters. Cluster I, consists of 6 genotypes and Siam Madu with 89% genetic similarity Cluster II consist of 14 genotypes with 91% genetic similarity. Cluster III consist of F17 and cluster IV consist of Satsuma Mandarin, with 58,6% and 24% genetic similarity, respectively. The genetic distance between all somatic hybrid genotypes with the parental Satsuma Mandarin is 0.60, except for F17 the genetic similarity is 0.34.