DOI: 10.36347/sjams.2020.v08i02.035

Pages: 533-540

Aim: The study aimed to investigate the antioxidant and anti-inflammatory effects of polydatin (PD) in liver preservation in an experimental early- and late-phase ischemic preconditioning (IP) model in rats. Materials and Methods: A total of 50 Wistar Albino rats were randomly divided into 5 equal groups. (I) The control group, received intraperitoneal saline injection only. (II) Early-phase IP (IE) group was formed at the end of second hour after the I/R procedure, (III) Early-phase IP + polydatin (IEP) group received intraperitoneal PD 40 mg/kg/day for 3 days after the I/R procedure, (IV) Late-phase IP (IL) group was formed at the end of day 3 after the I/R procedure, and (V) Late-phase IP + polydatin (ILP) group received intraperitoneal PD 40 mg/kg/day for 3 days and underwent hepatectomy at the end of day 3 after the I/R procedure. After blood and tissue sampling, all the rats were decapitated. Serum levels of Total antioxidant status (TAS), total oxidant status (TOS), alanine transaminase (ALT), aspartate transaminase (AST), hypoxia-inducible factor 1-alpha (HIF-1α), catalase (CAT), superoxide dismutase (SOD), and Glutathione Peroxidase (GSH-Px) in tissue samples were measured. Histopathological examination was performed using a light microscope. Results: CAT, SOD, and GSH-Px levels were increased in the PD groups (IEP and ILP) compared to the non-PD groups (IE and IL) and the increase in the CAT levels was statistically significant (p<0.05). HIF-1α levels were significantly lower in the PD groups compared to the non-PD groups (p<0.05). TOS levels were lower and TAS levels were higher in the PD groups compared to the non-PD groups although no significant difference was established in the two parameters (p>0.05). AST and ALT levels were lower in the PD groups compared to the non-PD groups and the ALT levels in the ILP group were significantly lower than in the IL group (p<0.05). Sinusoidal congestion, cytoplasmic vacuolization, polymorphonuclear leukocyte ......