DOI: 10.36347/sjavs.2015.v02i02.010

Pages: 125-130

Eugenol, the principal chemical component of clove oil, has been long well known for its local anesthetic, anti-inflammatory, antiseptic, and antibacterial effects. However, its anti-metastasis effect has not yet been fully explored. This study was performed to investigate the anti-metastasis effect of eugenol in human breast cancer cells (MCF-7). To ascertain the molecular mechanisms implicated in the anti-metastasis effect of eugenol on MCF-7 cell line was cultured and tests was performed using MTT assay, scratch-wound healing assay and evaluation transcriptional expression of MMP-9 and Paxilin expression by RT-PCR semi-quantification. Product percentage of relative density was calculated with Image J software. Data were analyzed with SPSS-16, ANOVA (P<0.05). Eugenol decreased cell viability dose and time dependently. MTT assay results represented that inhibitory concentration (IC-50 value) was 1.5µg/ml after 48h of incubation. Microscopic examination of eugenol treated cells showed cell shrinkage, membrane blebbing and apoptotic body formation. Scratch-wound healing assay which was conducted to investigate anti-migration effects of eugenol represented significantly inhibition of cell migration at 1.5 µg/mL (P<0.05)and suppressing metastasis ofMCF-7 cells. In this study we exanimated inhibitory effects of eugenol on downregulation of MMP-9 and paxilin mRNA expression which was remarkable with 34.3% and 13.7 reduction (P<0.05) in the gene expression. Taken together our finding may provide a new approach to use natural product for metastasis therapy and clinical use although further investigation are required.